The lawsuit follows weeks of feuding between the Republican administration and Democratic Gov. Janet Mills that has led to threats to cut off crucial federal funding and a clash at the White House when she told the president: “We’ll see you in court.”
Attorney General Pam Bondi announced the legal action at a news conference in Washington alongside former University of Kentucky swimmer Riley Gaines, who has emerged as a public face of the opposition to males in women's sports.
Trump's departments of Education and Health and Human Services have said the Maine agency is violating the federal Title IX antidiscrimination law by allowing transgender girls to participate on girls teams.
Maine officials have refused to agree with a settlement that would have banned male students from sports, arguing that the law does not prevent schools from letting males participate.
